Re: Manual or automatic staining
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From: | "Tony Henwood" <henwood@mail.one.net.au> |
To: | "'HistoNet Server'" <HistoNet@Pathology.swmed.edu>, "Todd Walker" <towalker@csu.edu.au> |
Reply-To: | |
Date: | Sun, 18 Apr 1999 13:48:17 +0000 |
Content-Type: | text/plain; charset=US-ASCII |
Dear Todd,
I suppose HE staining has two facets:
1. How the stain works
2. What problems can automatic staining machines give.
How the stain works would probably be the largest component in
learning about HE
How does Hx work, regressive or progressive, different ways in making
it up.
Different blueing solutions - why use them
Eosin solutions - alcoholic/aqueous, other red dyes that can be used
(phloxine, erythrosine, ponceau etc)
More importantly, how do you know when a HE is adequate for
diagnosis, whether it be manual or automatic. What controls to use.
What artifacts to look out for.
Manual staining allows the student to trial different techniques and
get a feel for the stain. Maybe following such scientific direction,
when they start work they may be able to scientifically control the
HE in their labs. From my experience at looking at HE from other
labs, some standardisation ( and ? improvement) would definitely not
go astray.
One question arises. What control best suits the HE stain? I use skin
(keratohyaline staining a good indicator for the Hx) coupled with
tongue (muscle, collagen etc shows the rich red hues that the
counterstain can give). Any discussion would be instructive.
Regards, Tony
Tony Henwood
www2.one.net.au/~henwood
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