Trisha,

The only "dumb or stupid" question is the question that was not asked!  All others
are valid and deserve replies.

Yes TUNNEL can be done on the BrdU stained slides, just be a bit carefull about
the 2 chromagens as both are determinations of neucular staining.



-- Begin original message --

> From: "P. Emry" <emry@u.washington.edu>
> Date: Tue, 27 Apr 1999 11:46:56 -0700 (PDT)
> Subject: Re: Double Staining
> To: Technical Services <techserv@dakousa.com>
> Cc: Cindy Chard-Bergstrom <CHARD_B@vet.ksu.edu>, Histonet@Pathology.swmed.edu
> 
> Hi,
> I have an over eager visiting fireman...he did Brdu on slides meant for
> apopotosis.  I am sure this is the dumbest question yet...but is it
> possible to re-stain or combine the two...ok...go ahead and laugh.  Wish I
> knew how to make this anonymous.
> Trisha
> On Tue, 27 Apr 1999, Technical Services wrote:
> 
> > Hi Cindy.
> > 
> > We have a double-stain kit at DAKO which utilizes our Envision polymer
> > system, and includes a blocking reagent that enables you to completely carry
> > out one stain, eliminate any vestige of it (aside from the colorimetric
> > reaction of course) and then proceed with another stain. You can use two
> > polyclonals, two monoclonals, what have you. I've spoken with people who
> > have even quadruple stained. If you would like more information please
> > contact me.
> > 
> > Thank you,
> > 
> > Joel Weisenberger
> > DAKO Corporation
> > Technical Services
> > 800-235-5743 x5325
> > techserv@dakousa.com
> > 
> > 
> > -----Original Message-----
> > From: Cindy Chard-Bergstrom <CHARD_B@vet.ksu.edu>
> > To: Histonet@Pathology.swmed.edu <Histonet@Pathology.swmed.edu>
> > Date: Tuesday, April 27, 1999 9:59 AM
> > Subject: Double Staining
> > 
> > 
> > >Dear Histonetters,
> > >
> > >    I will soon be working on a double staining IHC project. I seem
> > >to remember someone discussing a proceedure that involved stripping
> > >off the first primary, after addition of the chromogen, and then
> > >applying the second primary. Does anyone have this protocol or any
> > >others involving double staining. One project involves two rabbit
> > >primary antibodies and there is not enough antibody to conjugate
> > >either one to HRPO or alk-phos. Thanks to all in advance.
> > >
> > >Cindy Chard-Bergstrom, BS, HT(ASCP)
> Trisha Emry 
> U of Washington, Seattle
> 
> 
> 

-- End original message --

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone 
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**