Giemsa Stain

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From:"Sarah Christo" <>
Date:Tue, 27 Apr 1999 16:35:41 -0500
Content-Type:text/plain; charset=US-ASCII

Hello Netters,
  I'm posting this for Bill Fisher, any suggestions would be appreciated.  I have rosin powder (Fisher R-308, his procedure uses R-307) but was wondering if there isn't a procedure that doesn't require it at all.   -Sarah

>From Bill Fisher:
Dear Sarah,
Hi. As I mentioned on the phone I wanted to follow a procedure published by Askenase et. al. 1976, J.Immunol. 117:1721-1730 and used by this group for a number of years after to identify basophils in the skin of animals. They
used Giemsa stain on paraffin slides according to the method of McNamara as modified by Putt. In it they decolorized using colophonium(rosin, white lumps Fisher Sci. R-307). The white rosin is no longer available. Do you
have a procedure that decolorizes basophils in the skin of cattle or another procedure that works well with cattle skin for the identification of basophils and eosinophils? In the 1976 paper they were quite specific about the decorization of the basophils as too much decorization could render them indisquishable from eosinophils.

Any help along this line would be appreciated. 

Thanks again.

Bill Fisher

William F. Fisher
2700 Fredericksburg Rd
Kerrville, TX 78028

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

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