Does LacZ staining work on air-dried-cryosections?
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From: | "Masayuki Miyagishima, MD" <mmiyagis+@pitt.edu> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Sat, 17 Apr 1999 15:34:00 -0500 |
Content-Type: | text/plain; charset=iso-2022-jp |
Does LacZ staining work on air-dried-cryosections?
Has anyone tried it? If it works as good as non-air-dried cryosections,
I do not have to make any special modification for slide storage for
lacZ staining. Three days after the gene transfection, I kill the
animal and perfusion fix with 4% paraformaldehyde and snap-frozen in
Isopentan. I want to use these cryosection for all the IHC and possibly
for LacZ staining, too.
--
Masayuki Miyagishima, MD
University of Pittsburgh,
Department of Surgery
412-647-2345, and ask the hospital operator to page 3228, please.
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