Does LacZ staining work on air-dried-cryosections?

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From:"Masayuki Miyagishima, MD" <mmiyagis+@pitt.edu>
To:Histonet@Pathology.swmed.edu
Reply-To:
Date:Sat, 17 Apr 1999 15:34:00 -0500
Content-Type:text/plain; charset=iso-2022-jp

Does LacZ staining work on air-dried-cryosections?

Has anyone tried it?  If it works as good as non-air-dried cryosections,
I do not have to make any special modification for slide storage for
lacZ staining.  Three days after the gene transfection, I kill the
animal and perfusion fix with 4% paraformaldehyde and snap-frozen in
Isopentan.  I want to use these cryosection for all the IHC and possibly
for LacZ staining, too.

-- 
Masayuki Miyagishima, MD
University of Pittsburgh, 
Department of Surgery
412-647-2345, and ask the hospital operator to page 3228, please.



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