Re: sticky slides

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From:Kathy Wortham <>
To:"Masayuki Miyagishima, MD" <>, HistoNet Server <>
Date:Tue, 06 Apr 1999 08:02:39 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

Procedure for silane coated slides.

1-Place slides in staining racks
2-Place slides in acetone 2 minutes
3-Place slides in 2% silane solution 2 minutes
  (5ml silane to 250 ml acetone)
4-Distilled water 5 dips
5-Distilled water 5 dips
6-Distilled water 5 dips
7-Allow slides to air dry overnight
8-Store in slide boxes

I order the Silane from sigma. (3-aminopropyl-triethoxysilane 

I have tried commercially available silane and plus slides but they do
not work as well foe me and they are A LOT more expensive.

Kathy Wortham
USDA, Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605

--- "Masayuki Miyagishima, MD" <> wrote:
> Could you let me know how to make silane coated
> slide?  Are there any
> commercially availabld silane slide?  Is it better
> than superfrost plus
> slides?
> Masayuki Miyagishima, MD
> University of Pittsburgh, 
> Department of Surgery
> 412-647-2345, and ask the hospital operator to page
> 3228, please.


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