Procedure for silane coated slides.

1-Place slides in staining racks
2-Place slides in acetone 2 minutes
3-Place slides in 2% silane solution 2 minutes
  (5ml silane to 250 ml acetone)
4-Distilled water 5 dips
5-Distilled water 5 dips
6-Distilled water 5 dips
7-Allow slides to air dry overnight
8-Store in slide boxes

I order the Silane from sigma. (3-aminopropyl-triethoxysilane 
Cat#A-3648 

I have tried commercially available silane and plus slides but they do
not work as well foe me and they are A LOT more expensive.

Kathy Wortham
USDA, Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605
706-546-3556


--- "Masayuki Miyagishima, MD" <mmiyagis+@pitt.edu> wrote:
> Could you let me know how to make silane coated
> slide?  Are there any
> commercially availabld silane slide?  Is it better
> than superfrost plus
> slides?
> 
>
> Masayuki Miyagishima, MD
> University of Pittsburgh, 
> Department of Surgery
> 412-647-2345, and ask the hospital operator to page
> 3228, please.
> 

===

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