Thanks re:endogenous peroxidase, a serum blocking question

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From:Bruce A Rasmussen <>
Date:Tue, 06 Apr 1999 10:44:30 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

My thanks to all who wrote back with detailed responses to my endogenous
peroxidase question. If I could trouble folk with just one more question. 
I have a protocol that calls for a 5% serum block (30 min to the secondary
animal) before and primary and before the secondary. There is much debate
about whether the second block is necessay, if it will reduce the signal,
or if rinsing after the serum blocks is good or bad. Some folk also
suggest that a couple of percent BSA is a good idea.
Thanks again,

Bruce Rasmussen
Predoc Fellow in Experimental Neuropsychology
The Krasnow Institute for Advanced Study
George Mason University
Mail Stop 2A1
Fairfax, VA 22030-4444

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