Re: ? Re: PAS w/Digestion

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From:"J. A. Kiernan" <>
To:"Sarah A. Jones" <>
Date:Fri, 02 Apr 1999 23:19:50 -0500 (EST)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Fri, 2 Apr 1999, Sarah A. Jones wrote:

> Someone is telling me that there are folks out there doing PAS w/ and w/o
> Digestion on the same slide.  Has someone really developed a fool-proof
> technique for doing it this way, or is this person either uneducated or
> uninformed?  It doesn't make sense to me!

   It's quite easy. You mount at least 2 sections on the slide,
   not too close together. Apply a drop of amylase/spittle to
   one section; water to the other. Leave to digest, in suitably
   humid atmosphere to prevent evaporation. Wash well in water
   then stain the whole slide with PAS or some other method for

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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