Re: IHC endogenous peroxidase block
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| From: | "M. Brown" <marianb@u.washington.edu> |
| To: | Cynthia Favara <cfavara@niaid.nih.gov> |
| Reply-To: | |
| Date: | Fri, 02 Apr 1999 21:11:01 -0800 (PST) |
| Content-Type: | TEXT/PLAIN; charset=US-ASCII |
aSince 1992, I learned to block for endogenous peroxidase after the link
antibody.By doing this there is no destruction of the epitope.I started
doing this while working at OHSU, then continued this procedure with my
prostate cancer research projects.
Marianne
On Fri, 2 Apr 1999, Cynthia Favara wrote:
> Netters,
> Curious as to when people block for endogenous peroxidase. Before or
> after 1st/link antibody. Has anyone done any experiments for epitope
> destruction caused by the peroxidase block?
> Thanks for the input.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
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