From: | Gerard Spoelstra <spoelstr@numbat.murdoch.edu.au> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 09 Apr 1999 15:20:28 +0800 |
Content-Type: | text/plain; charset="us-ascii" |
Donna We've always made up our own Schiffs which is only an investment of 10-15minutes. In the method in Culling I always cool the reagent as quickly as possible and filter it at approx 55 degree C. Its made up with Pararosaniline. I use a kidney control section and use the basement membrane as an indicator of the quality of the Schiffs. When the basement membrane is no longer sharply defined and the Schiffs begins to stain indiscriminately then reagent is no longer viable. Gerard Spoelstra Histopathology School of Biomedical Science Murdoch University Western Australia