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From:Gerard Spoelstra <>
Date:Fri, 09 Apr 1999 15:20:28 +0800
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We've always made up our own Schiffs which is only an investment of
10-15minutes. In the method in Culling I always cool the reagent as quickly
as possible and filter it at approx 55 degree C. Its made up with
I use a kidney control section and use the basement membrane as an
indicator of the quality of the Schiffs. When the basement membrane is no
longer sharply defined and the Schiffs begins to stain indiscriminately
then reagent is no longer viable.

Gerard Spoelstra
School of Biomedical Science
Murdoch University
Western Australia

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