RE: Hales

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From:Alex Brown <>
To:"'Histonet'" <>
Date:Fri, 09 Apr 1999 13:42:00 +0100

Hi Mara,
	Also known as Hale's Dialysed Iron, the method is in Theory and
Practice  of Histological Techniques by Bancroft and Stevens.  We were
asked to do one last week for the first time in many moons. Must admit I
prefer Alcian blue, although the Hale's is supposed to be more
	a) Dialysed Iron
	b) 2M acetic acid
	c) 2% aqueous potassium ferrocyanide
	d) 2% hydrochloric acid
	1)  Equal parts of (a) and (b)				10 mins
	2)  Wash well in distilled water
	3)  Equal parts of (c) and (d)  { i.e. Perls' reagent }	10 mins
	4)  Wash well in distilled water
	5)  Counterstain e.g. with neutral red,  or you can tag on a PAS
as with Alcian Blue.
Note: 	Treating a duplicate section with the Perls' reagent only, will
exclude haemosiderin.
	The pH of the coloidal iron soution should be approx. 1.9

		Hope this helps,
			Alex Brown
			Crosshouse Hospital
			Kilmarnock, Scotland.

Subject: Hales
Date: 09 April 1999 01:51

Does anyone happen to have a procedure for the Hales Colloidal Iron
We don't have it in any of our (few) reference books.

Mara Fairman
Roper Hospital
Charleston, SC
Fax: 843-724-2356

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