Thank you all for answering my question. I think I need to
change our mounting medium first to see whether it helps.
Have a nice day!
On Wed Apr 16 07:37:59 EDT 2008, Piero Nelva
> We had a batch of mounting medium that went yellow, and
> consequently the section went yellow too (so long ago that I've
> forgotten the brand). Since switching to Eukitt, the preparation
> is much more stable.
> Piero Nelva
> Monash Medical Centre
> ----- Original Message ----- From: "FU,DONGTAO"
> Sent: Wednesday, April 16, 2008 6:21 AM
> Subject: [Histonet] background of H&E staining
>> Hi, all
>> Our PI complained about our H&E staining recently. He found the
>> slides we did for him changed color(within a month), the whole
>> slides turn to yellow, although the staining is still there and
>> looks good. I searched for some websites for the answer, but
>> could not find it.
>> Does anyone know the reason why the background of slides
>> changed color? We did it in the general way which worked well in
>> the past. And the reagents are not expired. Any suggestions?
>> Histonet mailing list
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> Histonet mailing list
Ann Dongtao Fu MD, Ph.D
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