Re: [Histonet] background of H&E staining

From:Rene J Buesa

This usually happens when washing is incomplete and some residual acid pH remains on the section that causes the fading over some time.
  René J.

"FU,DONGTAO"  wrote:
  Hi, all

Our PI complained about our H&E staining recently. He found the 
slides we did for him changed color(within a month), the whole 
slides turn to yellow, although the staining is still there and 
looks good. I searched for some websites for the answer, but could 
not find it.

Does anyone know the reason why the background of slides changed 
color? We did it in the general way which worked well in the past. 
And the reagents are not expired. Any suggestions?



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