We also use formic acid to decalcify our bone marrows. We do IHC on 2-3 bm cases a day with good results. Our CD34 is purchased from Becton-dickinson and used at a 1:200 dilution. Hier using citrate buffer for 20 minutes in a steamer, cool down for 10 minutes. 

Rena Fail

>>> "Gudrun Lang"  04/25/06 03:41PM >>>
Hi,
Can anyone share a protocol for CD34 on decalcified FFPE human bone marrow
biopsies?
We have a benchmark XT and decalcify with formic acid.
Are there any hints especially refering to CD34 in acid-decalcified tissue?

Thank you

Gudrun Lang


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