Re: [Histonet] Cd34 in trephine bone marrow
We use formic We use CD34 purchased from Becton-Dickinson. 1:200, citrate buffer for 20 minutes in the steamer. We also use formic acid to decalcify bone marrows.
Rena Fail
>>> "Gudrun Lang" 04/25/06 03:41PM >>>
Hi,
Can anyone share a protocol for CD34 on decalcified FFPE human bone marrow
biopsies?
We have a benchmark XT and decalcify with formic acid.
Are there any hints especially refering to CD34 in acid-decalcified tissue?
Thank you
Gudrun Lang
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