I am trying whole mount staining of mouse Retina, Trachea and 20 micrometer tissues with vWF (Rabbit anti human von Willibrand factor polyclonal antibody, Chemicon international) SMA (Monoclonal anti-actin, alpha smooth muscle antibody produced in mouse) and i need to use MOM kit (Vector) for this purpose.
   
  They recommend to incubate the samples for just 30 min with primary antibody.
  I wonder if this time is enough..................?? 
  Can anyone guide me a good protocol regarding this issue.
   
  Thanks
   
  Dr. Sohail

		
---------------------------------
Love cheap thrills? Enjoy PC-to-Phone  calls to 30+ countries for just 2¢/min with Yahoo! Messenger with Voice.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet