[Histonet] CD3 antibody for FFPE rabbit tissue
It's a wild guess and haven't tried it, but perhaps worthwhile to test
(....and better than nothing):
The LabVision CD3 rabbit monoclonal antibody (clone SP7) reacts very
well with a lot of different species (human, rat, mouse), so to my
opinion there is a good chance it reacts with rabbit CD3 as well.
Just test it on some lymphoid rabbit tissue and look through heavy
background staining (we have a rabbit-on-rabbit situation here and you
will observe a lot of plasmacells and staining near vessels!) whether
or not T-cells are positive. If this is the case you need the Zenon
(Molecular Probes/Invitrogen) rabbit-on-rabbit kit for proper
visualization. Please let us know if this approach works for you.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Thu, 27 Apr 2006 12:02:53 -0700
Subject: [Histonet] CD3 antibody for FFPE rabbit tissue
I am looking for a CD3 antibody to use on FFPE rabbit tissue. Most
antibodies I have looked into are tested in human.
Preclinical - Histology
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