Re: [Histonet] tendon processing
Buy the deeper metal molds for embedding oversize tissues. You can get
these from Fisher, VWR, Thermo, etc to fit your cassettes.
If you use high profile disposable blades, you get less chatter, more
stability during paraffin sections (tendons, decalcified bone, and very
fibrous tissues) . High profiles are just as sharp as low profile
disposable blades. Tendon may be a bit drier, so a gentle soak on warm
then cold water may help, beware of overdehydration and never add heat to
dehydration and clearing steps, that only dries out these tough tissues
even more. Propar should work fine and less hardening to this tissue or
any other tissue, just make sure you rotate this out more frequently as
this xylene substitute is a bit more sensitive to water carryover, xylene
tends to clear water a bit better, but keeping Propar fresh with more
frequent changes helps - this is dependent on how many tissues you put
through a processor in a week?
What do you mean by "charge more tendon"? or is that change knives more
often when sectioning tendon? You should be able to use disposable blades
rather than permanent c profile knives (if that is what you are using?)
At 10:00 AM 4/5/2006, you wrote:
>Please, will someone send a tried and true protocol for paraffin
>processing of tendon? These people won't trim the pieces thin enough and
>the cassettes sit up off the molds. The first few I used xylene and have
>since used Propar and they are that much better. I still could use any
>other helpful hints. Thanks muchly.
>Do you charge more tendon? I find I'm going through knives.
>Thanks for all your help.
>Mary Lou Norman
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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