RE: [Histonet] tendon processing

From:"Elizabeth Chlipala"

Mary Lou

We process quite a bit of human tendon samples and it can be
challenging.  The pieces we receive are not that thick but we process
between biopsy pads to help keep the tissue flat.  We use a longer
processing cycle (1 to 1.5 hours in each station).  When sectioning you
need to be very careful when rough trimming in so you don't tear the
fibers, let soak on cold ice for about 30 minutes to an hour and then
section.  We use new blades.  We place the cut sections flat on a warm
hot plate (paraffin does not melt) overnight and then stain the
following day.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Mary Lou
Sent: Wednesday, April 05, 2006 10:01 AM
Subject: [Histonet] tendon processing

Hello Histonet,

Please, will someone send a tried and true protocol for paraffin
of  tendon? These people won't trim the pieces thin enough and the 
cassettes sit up off the molds. The first few I used xylene and have
used Propar and they are that much better. I still could use any other 
helpful hints. Thanks muchly.
Do you charge more tendon? I find I'm going through knives.

Thanks for all your help.
Mary Lou Norman

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