Napththol  AS-D  Chloroacetate  Esterase  is used to identify granulo   cytes.    This   procedure   can  be  done  on  FFPE  tissue,or  blood
   smears.&nbs=  p;  Neutrophils  also  contain myeloperoxidase.  this is
   usually done on = blood smears.  Sigma has kits for both stains.



   <= DIV>Jennifer MacDonald
   -----histonet-bounces= @lists.utsouthwestern.edu wrote: -----

     To: Histonet@lists.utsouthwestern.edu
     Fr= om: "Webb, Dorothy L" 
     Sent by:= histonet-bounces@lists.utsouthwestern.edu
     Date: 04/04/2006 11:25AM
     S= ubject: [Histonet] neutrophils
     Does  anyone  know  of  a stain for neutro= phils?  I believe I had
     read that
     staining  with chloroacetate ester= ase is the way to go, but, I am
     not
     familiar  with  that  stain.   Does= anyone know of  a company that
     would
     have  the  stain  in a kit?? &nb= sp;This is for a research project
     and am rather
     stumped!   Thanks,  a=  s always, fellow Histonetters for your help
     and
     support!!!!
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