Re: [Histonet] Hematoxylin staining of frozen samples, where are the nuclei?
Tissues go through a long procedure with immuno and the most detrimental for
all tissue components is the heat retrieval and to a lesser degree enzyme=20
digestion. The less well fixed and processed tissue suffers most: connective
tissue is distorted and nuclei stain very poorly.
Hope this explains your problem,
Hamilton, Ontario, Canada
----- Original Message -----
From: "Guillermo Palao"
Sent: Tuesday, April 04, 2006 5:22 PM
Subject: [Histonet] Hematoxylin staining of frozen samples,where are the
> Dear histonetters,
> When I stain samples placed on VWR frosted slides with Gills hematoxylin,
> nuclei are nicely stained. However, when the same samples are
> counterstained after passing through all necessary steps involved in
> immunohistochemistry, hematoxylin staining of nuclei is simply horrible.
> Is it possible that nuclei are lost in the different washing steps? Please
> put forward any suggestions you might have.
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