one time on The X-files Scully had 4 hours until a meeting and she needed to 
prove she was infected with the alien virus at the meeting.  She took her 
own blood, purified lymphocytes, extracted DNA, digested the DNA, ran a gel, 
transferred the gel to a filter to which she hybridized a "really hot probe" 
that she had also managed to synthesize/purify (not clear) and label while 
the gel was running and transferring, then washed the blot and exposed it to 
film and got to the meeting with the data.  I am still trying to recover 
from that bastardization of science (and that has been quite a few years 
now) so in the meantime I simply watch House because of Hugh Laurie (I 
digress from this digression but the actor who plays his friend Wilson 
doesn't get the kudos he deserves for playing his character)  although one 
of my colleagues is totally torn up over the fact that House uses his cane 
in the wrong hand ;-P
whew!  ok, back to reality.
dm

----- Original Message ----- 
From: "Shirley Powell" 
To: "'Linda Blazek'" ; 
; 
Sent: Thursday, April 13, 2006 1:40 PM
Subject: RE: [Histonet] Anyone catch "House" the other night?


>
> Turn off the TV get in the Jacuzzi, have a glass of wine and relax.
> Work is work, TV is TV and they get paid a lot more than we do for doing
> histology wrong.  Go figure.
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda 
> Blazek
> Sent: Thursday, April 13, 2006 11:38 AM
> To: Histonet@lists.utsouthwestern.edu; lpjones@srhs-pa.org
> Subject: Re: [Histonet] Anyone catch "House" the other night?
>
> My husband turned to me as said "I thought you said doing immunos was a
> complicted thing."
>
>>>> "Jones, Laura"  4/13/2006 12:27 PM >>>
>
> Dr. House and his colleagues were doing immuno on Tuesday night! 
> Procedure:
>
> Take large chunk of heart tissue which has not been frozen or in fixative,
> but in a petri dish in the fridge, and flop it on to your microscope 
> slide.
> Put the slide with the  large chunk of tissue on it under the scope.  Add
> "one microlitre of immunoperoxidase" to the tissue.  Look into scope.  If 
> it
> turns red, it's positive.
>
> I wish our immuno were that easy and quick.  Get a Histo consultant on
> staff, please!  :o)
>
> P.S. And how about those doctors who do biopsies, ultrasounds, draw blood,
> and then test it all?  In evening gowns and tuxes?
>
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