RE: [Histonet] Tissue graft coming off of slide

From:"Monfils, Paul"

For sections of synthetics I usually use a chrome alum gelatin product in
the water bath.  I used to make my own but in recent years have relied upon
"Sta-On", manufactured by Surgipath. You have two major problems facing you.
First, the need for antigen retrieval.  Most of my work involving synthetic
materials has not required antigen retrieval.  The Sta-On has worked very
well for me in most cases but I'm not sure how well it will stand up to
boiling.  Secondly, the particular synthetic you are working with, PTFE
(polytetrafluoroethylene), which is marketed under the familiar trade name
"Teflon", is specifically designed not to stick to anything!  Still, I have
had pretty good success with PTFE implants by using a fairly high
concentration of Sta-On in the water bath, followed by drying on an 80
degree slide warmer before overnight drying at 60 degrees - but again,
without antigen retrieval.

> ----------
> From: on behalf of
> Reply To:
> Sent: 	Monday, April 3, 2006 11:46 AM
> To:
> Subject: 	[Histonet] Tissue graft coming off of slide
> Just wondering if anyone has any ideas to keep ePTFE (I will ask
> researcher what this stands for)
> graft tissue from porcine from coming off of slides during antigen
> retrieval.  Actually, it is
> starting to come off even after deparaffinization.  I told the researcher
> this is our last hope
> that someone on the Histonet might have an idea.  Here is a summary of
> what I have tried.  I used
> silanized slides (from Dako) with an overnight bake at 57C for tissue
> adherance plus an additional
> 10 minute 10% NBF soak to fix the tissue firmly on the slide after
> deparaffinization and before
> antigen retrieval.  I have used low temperature antigen retrieval (LTAR)
> in the past for delicate
> tissue, but if the tissue comes off after deparaffinization what is the
> point.  Is there something
> else out there that I can try?  Thanks for any and all help.
> Bob Meyer
> Sr. Research Technologist
> Northwestern University
> Chicago, IL  60445
> 312-908-5546
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