RE: [Histonet] PE Tumor tissue; Transwell embedding

From:"Elizabeth Chlipala"


We have processed millipore membrane inserts.  We just processed them
routinely to paraffin.  We wrote a article for Sakura Histologic about
it.  The link is below:


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of
Makhijani, Nalini S
Sent: Friday, April 14, 2006 1:18 PM
Subject: [Histonet] PE Tumor tissue; Transwell embedding

Hi Histonetters,

I have 2 questions:


1. Does anyone have an opinion on the correct disposal method for
paraffin embedded tumor tissue?


2.  Does anyone have experience with paraffin embedding, sectioning and
H&E staining of Transwell membrane inserts, either Corning Costar, or

I've been following the protocol from the Corning website, using
Citrisolv from Fisher instead of Histoclear.

The sections of the membranes are thin lines about 3mm long.

After about 2 min in Citrisolv the sections look so clear that it's not
clear if they are on or not.  If  they are, they are usually lost in the
first absolute alcohol.  After staining, nothing is visible.  Do these
membranes, without cells, stain or remain transparent?

Are 3mm sections too small to remain on Superfrost Plus slides?  Should
the slides be subbed?  Does anyone else have a problem getting
transwells and cells to stick to the slides?




VAMC, West Los Angeles


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