You can stain with the Von Kossa with a nuclear fast red counterstain.
Ruth Yaskovich
N.I.H.


> ----------
> From: 	Jennifer MacDonald
> Sent: 	Monday, April 18, 2005 2:18 PM
> To: 	Gary Radice
> Cc: 	Histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu
> Subject: 	Re: [Histonet] neutral stains for protozoa
> 
> There is an iron hematoxylin procedure for protozoa.  It is in the AFIP 
> manual.  I don't have it.  It is Heidens or something similar.
> 
> 
> 
> 
> 
> 
> Gary Radice  
> Sent by: histonet-bounces@lists.utsouthwestern.edu
> 03/31/2005 06:47 AM
> 
> To
> Histonet@lists.utsouthwestern.edu
> cc
> 
> Subject
> [Histonet] neutral stains for protozoa
> 
> 
> 
> 
> 
> 
> I have a colleague who is studying a calcified protozoan. She is 
> specifically interested in calcification, and she is looking for stains 
> she can use that are not acidic and that don't require acid 
> differentiation steps, so that she doesn't inadvertently decalcify the 
> specimens.
> 
> Any thoughts about nuclear or counterstains that might work in this 
> situation?
> 
> 
> 
> Gary P. Radice gradice@richmond.edu
> Department of Biology            804-289-8107 (voice)
> University of Richmond           804-289-8233 (FAX)
> Richmond VA 23173                http://www.richmond.edu/~gradice
> 
> 
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