There is an iron hematoxylin procedure for protozoa.  It is in the AFIP 
manual.  I don't have it.  It is Heidens or something similar.






Gary Radice  
Sent by: histonet-bounces@lists.utsouthwestern.edu
03/31/2005 06:47 AM

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Subject
[Histonet] neutral stains for protozoa






I have a colleague who is studying a calcified protozoan. She is 
specifically interested in calcification, and she is looking for stains 
she can use that are not acidic and that don't require acid 
differentiation steps, so that she doesn't inadvertently decalcify the 
specimens.

Any thoughts about nuclear or counterstains that might work in this 
situation?



Gary P. Radice gradice@richmond.edu
Department of Biology            804-289-8107 (voice)
University of Richmond           804-289-8233 (FAX)
Richmond VA 23173                http://www.richmond.edu/~gradice


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