[Histonet] pretreat for her2neu

From:Dawn Cowie

hello histonetters,
I'm hoping someone can help me with this. We are using the ventana benchmark for all our immunos. The benchmark calls for using the cell conditioning 2 as a pretreatment for her2neu. It is very expensive (about 950.00 for 1 litre) It only has a shelf life of 2 months.
Our problem is that it is expiring before we can use hardly any of it. We are looking at trying to use cell marques Declere as the de paraffinization and pretreat step off line and then continuing with the protocol on the benchmark from that point on. The Cell condition 2 is a citrate buffer, as is the Declere. My question is, has anyone out there who is using benchmark had any experience of this or of using another alternative?
Any help would be appreciated.
Dawn Cowie HT (ASCP)
Pensacola Path
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