[Histonet] Oozing mounting medium

From:Gayle Callis


I have had this happen and one needs to do longer drying, it happened with
both xylene and toluene based mounting media.  Been there and experienced
your problem with gigantic sheep tibia and human femoral bone sections.   

The problem is with that large section in fact, everything is BIGGER!  Even
though you think section is truly flat, it often had thick thin areas, more
so than routine, small paraffin section.     

Don't try to overclean these slides, this removes enough media to cause an
air bubble particularly if you use dipping into xylene, then wiping to
clean.  This dissolves enough media away to create air bubbles.    It also
sounds as though longer is needed, these are huge sections with a lot of
goo under that coverslip.  

If the bubble connects to outer edge of coverslip, use a very fine pipette
to add media at bubbble edge and let it flow into bubble space, redry. We
have repaired bubbles this way before.  A #2 thickness coverslip is often
nicer. One could also try a weight in middle of section to keep large
coverslips flat.  

At 08:06 AM 4/22/2004 -0400, you wrote:
>Hello Histonetters
>My 5 x 7 inch slides are air dried flat for at least a month before I file
>them upright.  The problem I'm having is that once the slides are filed some
>of the toluene based mounting medium oozes out.  After I clean the slides
>off there is usually a big air bubble where the tissue is and I don't like
>to re-coverslip for fear of the tissue falling off since it has happened.  I
>spoke to a sales rep and he is sending me different mountants to try ( I
>assume all xylene based since I coverslip from xylene) but I was wondering
>what everyone else uses to prevent this from happening.   Any suggestions to
>eliminate this problem?  
>Thanks for your help.
>Peggy DiCarlo HT (ASCP)
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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