RE: [Histonet] Help with frozen section staining

From:"Barry R Rittman"

Pierre et al
Hi to y'all in Canada, I have a relative in Brampton, Ontario and really enjoy my visits there.
A point re OCT compound. It contains several salts including I believe some calcium. It is important therefore for some procedures such as when using certain lectins to thoroughly remove the OCT from the section to prevent interference with staining and with other procedures. This is easily taken care of with buffer/or alcohol etc.

-----Original Message-----
From: [] On Behalf Of Greg Dobbin
Sent: Friday, April 02, 2004 3:02 AM
Subject: Re: [Histonet] Help with frozen section staining

Hi Pierre-André (neighbor),
I don't see the OCT being the most likely culprit, unless MAYBE if 
you were perfusing with it. The OCT should only be surrounding 
your tissue sections (not entering into them).  I would be more 
inclined to look at:
a) the fixative (try brand new batch, if the fixative is faulty, your 
sections will not tolerate the non-isotonic and perhaps acid or basic 
pH of your staining solutions). And then
b) the mounting medium (for clarity) and the coverslips for defects. 
(presumably you've ruled out the microscope as the problem?) Good luck. Greg

Date sent:      	Fri, 02 Apr 2004 08:28:01 -0400
From:   (Pierre Noel)
Send reply to:
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Subject:        	[Histonet] Help with frozen section staining

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> Hi all,
> We just recently notice a major problem with our frozen section 
> staining. The cellular morphology is poor, haziness and no cellular 
> definition. We have tried different staining techniques with different 
> fixative but no luck. Could it be our OCT? We desperately need some 
> input on the subject. Thank you!
> Pierre-André Noel
> Histology Supervisor
> Bathurst Regional Hospital
> NB, Canada
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
Making a living is getting; making a life is giving.

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