RE: green PAP stains

From:Gary Gill

It's not possible to generalize about EA formulations, as all are different.
Papanicolaou changed his formulations and didn't describe them in
quantifiably reproducible terms (1942, 1954, 1960).  Manufacturers do their
own thing.  EA-50 is a commercial numerical designation; Papanicolaou did
not describe such a formulation.  Bismarck brown precipitates
phosphotungstic acid and compromises differential staining by light green
and eosin.  The list goes on ...

Gary Gill

-----Original Message-----
From: Carlos Defeo []
Sent: Tuesday, April 15, 2003 1:09 AM
Subject: Re:green PAP stains

EA 50 has an important  proportion of Light Green (this is  even more
dangerous if fast green FCF is employed instead of light green),so the ratio
light green/eosin+bismark brown is high. EA 65 has a more rational
composition, including  half of the amount of light green and giving then a
more precise coloration.
Try the other batch but it looks like there is an irrational formulation of
Carlos ,Montevideo-Uruguay.

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