Is anyone working with human interferon Beta on paraffin, rat brains? What
antibody are you using? Protocols? Anything?
Annette

-----Original Message-----
From: John Auld [mailto:John.Auld@whnt.nhs.uk]
Sent: Wednesday, April 23, 2003 04:13
To: HistoNet Server
Subject: Re: Coverslipping and gloves



It's suddenly got a bit heated, anyway without taking sides. I recollect
being told at a safety seminar, sorry no references etc, that although
nitrile gloves do not dissolve in xylene they are NOT resistant. The
presenter said nitrile allowed xylene to penetrate after a very few
minutes, forget the exact figure but it's no more than one or two. The only
safe way IMHO is to use an automated coverslipper.

Regards

John

John Auld FIBMS MSc
Dept of Histopathology and Clinical Cytology
Arrowe Park Hospital
Arrowe Park Road
Upton
Wirral
UK