I have used anti-F4/80 on mouse CNS fixed with 4% paraformaldehyde 
perfusion, snap frozen and cut at 20 microns on a sliding microtome. I 
ran the primary antibody at 1:1000 or 1:2000 all night at room temp. on 
a shaker table (very gentle agitation) then used a Vector ABC Elite kit 
and DAB intensified with either nickel + cobalt or Vector's "Intense" 
reagent.  Beautiful results.
    Clint Lincoln on this list has done F4/80 on FFPE sections after 
enzyme treatment.

Geoff

Sylene.Goodwin@UCHSC.edu wrote:

>Has anyone used this on frozen mouse tissue?  Particularly mouse kidney?  What type of fixation did you use?  Caltag's technical support and references are not exceptionally helpful.
>
>Thank-you!
>
>Sylene
>
>  
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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