RE: human Ab on human

From:Chris van der Loos

I fully agree with Scott Turners's explanation that an anti-mouse reagent 
will not find any binding sites to a "humanized" antibody. Unfortunately, 
there is no commercial human "ARKit-like" system available. At least not to 
my knowledge.
The reference Nick Bulough was referring to is probably Tuson et al. (1990) 
J Histochem Cytochem 38:923-926: "A Novel immunohistochemical technique for 
the demonstration of specific binding of human monoclonal antibodies to 
human cryostat tissue sections". Except for the fact it didn't come from 

Chris van der Loos,
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands

Joyce Judge wrote:
 >Date: 11 Apr 2003 06:15:45 -0600
 >From: joyce judge 
 >Subject: human Ab on human
 >I have gone through the histonet archives to look for answers regarding
 >Human Ab on Human tissue. I was wondering how people have resolved this
 >issue. I will need to do IHC on a "humanized" antibody in human tissue and
 >we can not biotinylate or conjugate the primary at all. Can anyone offer
 >any help?
 >Joyce Judge

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