Re: "Who is the first"
Denne meldingen inneholder ikke sensitiv informasjon som bryter med
Rikshospitalets krav til informasjonssikkerhet.
(In compliance with the Security Policy of Rikshospitalet , the content of
this message (including any attachments) is of a non-sensitive nature)
Using 1% or greater H2O2 to block endogenous peroxidase has a detrimental
on the epitope recognised by anti CD4 clone 1F6 (ref. datasheet).
NC recommends blocking with 0.5% H2O2 before unmasking the antigen,
but in my hands, best results with this antibody were obtained when
peroxidase was blocked after incubation of primary antibody
(higher concentrations of H2O2 may then be used)
Please take a look at "Blocking Peroxidase", Discussion Board at
http://home.no.net/immuno or go there directly at:
Hogne Roed Nilsen
Date: 12 Apr 2003 22:10:59 -0600
From: IHC World
Subject: Re: "Who is the first"
RE: "Who is the first"In theory, blocking of endogenous peroxidase activity
can be applied at any step BEFORE incubation of HRP-conjugated antibody. I
usually do the blocking step after HIER/PIER, but have been testing it
HIER/PIER. No difference was observed. So I think the key is to be
cross all experiments.
----- Original Message -----
From: Tony Henwood
To: 'Fernando Capela e Silva' ; HISTONET@pathology.swmed.edu
Sent: Wednesday, April 09, 2003 4:43 PM
Subject: RE: "Who is the first"
We block endogenous peroxidase (Methanol-H2O2) before HIER or enzyme
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
From: Fernando Capela e Silva [mailto:email@example.com]
Sent: Wednesday, 9 April 2003 18:31
Subject: "Who is the first"
In immunohistochemistry "who is the first"?
Pre-treatment (HIER or enzymatic digestion) of tissues and after hydrogen
peroxide to eliminate endogenous activity
Hydrogen peroxide to eliminate endogenous activity and after pretreatment
(HIER or enzymatic digestion) of tissues
I already see both procedures. Is not important the order?
* * * * * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laborat#243#rio de Biologia da Conserva#231##227#o
Departamento de Biologia
Universidade de ...vora
Phone: +351-266 760 800
Fax: +351-266 711 231
<< Previous Message | Next Message >>