Recipe for Neutral Buffered Ethanol

From:Dave Allsop

Hi

I have whole fish preserved in 10% NBF from my recent field visit. I now
need to transfer them to 60-70% ethanol for storage, but need to buffer the
ethanol to pH 7-8, using sodium phosphate (I cannot use a calcium chelator
as I am interested in the calcified ear bones as well as the gonad
structure). If the ethanol is not buffered the ear bones may be dissolved.
Does anybody have a recipe for how to make such a Neutral Buffered Ethanol
Solution?

By the way, thanks to all those who helped with my previous question
regarding optimal preservation of gonads and otoliths (fish ear bones) in
the field.

many thanks

Dave Allsop
-- 
Dave Allsop
Ashworth Laboratories
Institute of Cell, Animal and Population Biology
University of Edinburgh                             X>   X>
Kings Buildings                             X>    X>
West Mains Road                                                 X>
EH3 9JT
Scotland

Email: David.Allsop@ed.ac.uk
Office Phone: (0131) 650 8667
Home Phone: (0131) 667 4423
WWW: http://homepage.mac.com/armydave/Frontpage.htm








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