Recipe for Neutral Buffered Ethanol

From:Dave Allsop


I have whole fish preserved in 10% NBF from my recent field visit. I now
need to transfer them to 60-70% ethanol for storage, but need to buffer the
ethanol to pH 7-8, using sodium phosphate (I cannot use a calcium chelator
as I am interested in the calcified ear bones as well as the gonad
structure). If the ethanol is not buffered the ear bones may be dissolved.
Does anybody have a recipe for how to make such a Neutral Buffered Ethanol

By the way, thanks to all those who helped with my previous question
regarding optimal preservation of gonads and otoliths (fish ear bones) in
the field.

many thanks

Dave Allsop
Dave Allsop
Ashworth Laboratories
Institute of Cell, Animal and Population Biology
University of Edinburgh                             X>   X>
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West Mains Road                                                 X>

Office Phone: (0131) 650 8667
Home Phone: (0131) 667 4423

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