Re: c-kit (CD117)
DAKO has a nice polyclonal c-kit. It works with no retrieval at all at
1:200. We do use Envision on it though since these tumors usually have a lot
of biotin in them.
----- Original Message -----
From: "Klassen, Shannon SDH"
Sent: Thursday, April 18, 2002 11:55 AM
Subject: c-kit (CD117)
> Can someone out there in histoland help me get this antibody working -
> Novocastra ckit clone T595 - I'm not getting membrane staining - some
> cytoplasmic and some almost appears nuclear. I've tried a variety of
> different tumors - GIST, melanoma, breast , normal skin and haven't had
> Down to 1\10 with antigen retrieval in citrate buffer in microwave.
> Shannon Klassen
> IHC Tech
> Saskatoon Health District
> Saskatoon, Canada
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