Collagen Stains??

From:Boneslides@aol.com

Can anyone recommend a stain that will differentiate different types of 
fibro-cartilage?  I am trying to differentiate the nucleus pulposis from 
annulus fibrosis in spine sections.  My understanding (which is VERY 
limited!!!) is that one is made of Type I collagen and the other is Type II 
collagen.

Any help will be greatly appreciated!

Thanks!
Diane Mahovlic
Orthopedic Pathology and Biomaterials Laboratory
The Cleveland Clinic Doundation
Cleveland, Ohio




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