Re: Breast specimens
Ah.The old stew meat vs. lean cuisine!..When we get
spec.that are unfixed..like the ones you have..we run them back thru the
processor on clean cycle..than return to formalin..and put back on
processor with the normal run.I realize it delays results a day..But
hey..They cant read what they submitted anyway..you could try 70% alcohol ..let
the fatty tissue sit in it until processing. You will just have to let the new
residents know..if you do this..to big ect..that's fine..but you wont have
results for a couple days.Good Luck!
----- Original Message -----
Sent: Monday, April 22, 2002 6:16
Subject: Breast specimens
Could we please revisit my current nemesis, which
is surely going to drive me into leaving Histology forever and seeking a
lucrative highly paying successful career?
help/advice/suggestions regarding handling breast specimens so that
they are well preserved, easy to cut on the microtome, instead of partially
raw tissue which requires the cut and scoop method to obtain a slide.
I plead with the residents (1st yrs.) to trim
them so that they fit in the cassette without touching the sides, and cut them
to 2mm in thickness. Of course, most times they are 4mm or more. They are then
placed into cassettes and held overnight in 10%NBF. They get put on the
processor the next evening. I tried alcoholic formalin, saw no difference.
Large breast lymph nodes are just as bad if not worse, raw in the centers. The
small ones are usually fixed O.K. Does anyone have a tried and true method for
handling large breast specimens which works for them with few exceptions? If
so, please share it, I will be forever in your debt.
Connie L. Probert
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