----- Original Message -----
Sent: Monday, April 22, 2002 6:16
PM
Subject: Breast specimens
Could we please revisit my current nemesis, which
is surely going to drive me into leaving Histology forever and seeking a
lucrative highly paying successful career?
I need
help/advice/suggestions regarding handling breast specimens so that
they are well preserved, easy to cut on the microtome, instead of partially
raw tissue which requires the cut and scoop method to obtain a slide.
I plead with the residents (1st yrs.) to trim
them so that they fit in the cassette without touching the sides, and cut them
to 2mm in thickness. Of course, most times they are 4mm or more. They are then
placed into cassettes and held overnight in 10%NBF. They get put on the
processor the next evening. I tried alcoholic formalin, saw no difference.
Large breast lymph nodes are just as bad if not worse, raw in the centers. The
small ones are usually fixed O.K. Does anyone have a tried and true method for
handling large breast specimens which works for them with few exceptions? If
so, please share it, I will be forever in your debt.
Connie L. Probert
Detroit,
Michigan