Good Morning all, happy spring....
Anyone know of any articles published concerning the effects of
decalcification on antigenicity of tissue used for immuno?
I am running Ki67 (mib-1) on osteosarcomas and chondromas and there seems to
be a loss of positive staining on most, but not all of them. I suspect that
the decalcification process has effected them. 
thanks
Jim Fessler
Pennsylvania Hospital
Philadelphia, Pa