Could we please revisit my current nemesis, which
is surely going to drive me into leaving Histology forever and seeking a
lucrative highly paying successful career?
help/advice/suggestions regarding handling breast specimens so that
they are well preserved, easy to cut on the microtome, instead of partially raw
tissue which requires the cut and scoop method to obtain a slide.
I plead with the residents (1st yrs.) to trim them
so that they fit in the cassette without touching the sides, and cut them to 2mm
in thickness. Of course, most times they are 4mm or more. They are then placed
into cassettes and held overnight in 10%NBF. They get put on the processor the
next evening. I tried alcoholic formalin, saw no difference. Large breast lymph
nodes are just as bad if not worse, raw in the centers. The small ones are
usually fixed O.K. Does anyone have a tried and true method for handling large
breast specimens which works for them with few exceptions? If so, please share
it, I will be forever in your debt.
Connie L. Probert
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