Re: Trouble Shooting
Responding to the message of
from "DeLovino, Salvacion S." :
Run your tissue sections back through xylenes, and alcohols and rerun on a
normal processing schedule. May not be "perfect" (might still have some brittle
edges)...but should be salvageable. Good Luck.
> Hello Histonetters,
> We had a problem with our tissue processing last week and until now,
> they are still arguing about what should and shouldn't have been done. What
> happened was, our MLA used methyl alcohol instead of xylene when she changed
> the solutions. So it was methyl alcohol instead of xylene right before
> paraffin. It was her fault for not reading the label but couldn't really
> blame her as the containers look alike. Anyways, we didn't know at first
> that that was what happened but we noticed the tissues were hard and brittle
> on the edges but soft in the middle. Was there something we could have done
> to save the tissues? Please help us settle the arguments!
> Thanks a lot in advance.
> Salvacion S. Delovino
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