RE: Prostate core biopsies
We supply the Dr.'s with Omni-Fix. (an alcoholic fixative) They are then
processed with NBF. We use Enzo's K903 AB and strong trysin digestion.
From: Carpenter, Judith A. [mailto:Jude.Carpenter@vtmednet.org]
Sent: Tuesday, April 09, 2002 11:10 AM
Subject: Prostate core biopsies
We're having trouble getting good nuclear staining and K9 IHC staining on
our prostate needle biopsies.
Clinician's offices fix them in 10% NBF. We put through 4 hour process with
zinc formalin in the first couple of stations as this enhances the nuclear
staining on our other tissues
fixed in 10% NBFbut not the prostate bxs for some reason.
Can you folks share what fixation gives you excellent H&E staining and what
K9 antibody you use
for IHC (our HIER is steam and we use Proteinase K pretreatment) ?
Appreciate your help-
Jude Carpenter,BS, HTL(ASCP)
Chief Technologist/Surgical Pathology/Histology/Autopsy
111 Colchester Ave.
Burlington, VT 05401
FAX : (802)847-3509
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