We supply the Dr.'s with Omni-Fix. (an alcoholic fixative) They are then
processed with NBF. We use Enzo's K903 AB and strong trysin digestion. 

-----Original Message-----
From: Carpenter, Judith A. [mailto:Jude.Carpenter@vtmednet.org]
Sent: Tuesday, April 09, 2002 11:10 AM
To: 'NSH'
Subject: Prostate core biopsies


We're having trouble getting good nuclear staining and K9 IHC staining on
our prostate needle biopsies.
Clinician's offices fix them in 10% NBF. We put through 4 hour process with
alcoholic
zinc formalin in the first couple of stations as this enhances the nuclear
staining on our other tissues
fixed in 10% NBFbut not the prostate bxs for some reason.
Can you folks share what fixation gives you excellent H&E staining and what
K9 antibody you use
for IHC (our HIER is steam and we use Proteinase K pretreatment) ?
Appreciate your help-
Thanks-
Jude
Jude Carpenter,BS, HTL(ASCP)
Chief Technologist/Surgical Pathology/Histology/Autopsy
FAHC/MCHV Campus
111 Colchester Ave.
Burlington,   VT  05401
(802)847-5116
FAX : (802)847-3509
jude.carpenter@vtmednet.org