RE: Immunohistochemistry

From:"Morken, Tim"

Solutions of  powdered milk have been used (the protein casien (sp?)blocks
non-specific binding)and some companies sell their own proprietary
protein-free blocking agents. 

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Miller, Barbara []
Sent: Friday, April 12, 2002 8:48 AM
To: ''
Cc: Miller, Barbara
Subject: Immunohistochemistry

Does anyknow of blocking agents other than serum or albumin that would
provide a low background for immunohistochemistry.   We are using a
fluorescent  dye (often Texas Red) and background is a problem so far.

Thank you for your help.

Bobbi Miller
Department of Gastroenterology
Roudebush Medical Center
Indianapolis, IN


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