Solutions of  powdered milk have been used (the protein casien (sp?)blocks
non-specific binding)and some companies sell their own proprietary
protein-free blocking agents. 

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Miller, Barbara [mailto:bmiller2@iupui.edu]
Sent: Friday, April 12, 2002 8:48 AM
To: 'histonet@pathology.swmed.edu'
Cc: Miller, Barbara
Subject: Immunohistochemistry



Does anyknow of blocking agents other than serum or albumin that would
provide a low background for immunohistochemistry.   We are using a
fluorescent  dye (often Texas Red) and background is a problem so far.

Thank you for your help.

Bobbi Miller
Department of Gastroenterology
Roudebush Medical Center
Indianapolis, IN

e-mail:  bmiller2@iupui.edu