Re: Cox-1 and Cox-2
From: | r-meyer2@northwestern.edu |
Dana,
I have worked with the Cox 2 monoclonal mouse antibody on FFPE human tissue
from Cayman Chemical, Ann Arbor, MI (Cat. # 160112; 800-364-9897). I used
colon adenocarcinoma as a control with a dilution of 1:100. For pretreatment,
I used citrate buffer pH 6.0 (DAKO 10X Target Retrieval Solution, S1699) for 20
minutes in a steamer when the buffer had reached above 95 degrees celsius, and
a 20 minute cool down at room temperature. I used DAKO EnVision + detection
system -- peroxidase block (DAKO S2001) for 10 minutes, protein block (X0909)
for five minutes, primary ab for 30 minutes, anti-mouse secondary ab/HRP
polymer for 15 minutes, and DAB/H2O2 for 7 minutes. The staining is excellent
and everyone here was really happy with the results.
Bob Meyer, HTL
Pathology Core Facility
Northwestern University
Dana Settembre writes on Thu, 11 Apr 2002 15:37:28 -0400:
>
>
> Hello,
> I'd like to run Cox-1 and Cox-2 on FFPE human tissue. Can't seem to get it
to work.
> Which vendor do you use? What about control? Pretreatment? Dilution?
Help!
>
> Thank you,
> Dana Settembre
> University Hospital-UMDNJ
> Newark, NJ
>
>
>
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