Dear Charles, There is a big difference between Pop-Tarts and chloroform. I
understand we all live and work with a certain amount of risk, however I
feel some things should be eliminated from our work environments if
possible. We use a B-5 substitute for our lymph nodes. I can't say if it is
a revealing solution or not, but it works for us.

Thomas Jasper HT(ASCP) BAS
Histology Team Leader
SMDC Clinical Laboratory
Duluth, MN
jaspert@smdc.org



	-----Original Message-----
	From:	Charles.Embrey [SMTP:Charles.Embrey@carle.com]
	Sent:	Tuesday, April 24, 2001 8:30 AM
	To:	't.hacker@har.mrc.ac.uk'
	Cc:	'histonet@pathology.swmed.edu'
	Subject:	RE: lymph node revealing solution

	I'm sure the Pop-Tart I had for breakfast wasn't good for me either.
Lets
	face it most of the chemicals we use (including water) will
eventually be
	listed as hazardous.  Each time we boot a beloved chemical out our
job
	becomes just a little harder.  Besides I think the Chloroform smells
neat.
	(strickly a personal opinion.....)
	Charles R. Embrey Jr., PA(AAPA), HT(ASCP)
	Histology Supervisor
	Carle Clinic
	Urbana, IL



	-----Original Message-----
	From: t.hacker@har.mrc.ac.uk [mailto:T.Hacker@har.mrc.ac.uk]
	Sent: Tuesday, April 24, 2001 3:13 AM
	To: Charles.Embrey
	Cc: 'Histonet'
	Subject: RE: lymph node revealing solution


	Date sent:      	Mon, 23 Apr 2001 15:59:06 -0500
	From:           	"Charles.Embrey" <Charles.Embrey@carle.com>
	Subject:        	RE: lymph node revealing solution
	To:             	"'LINDA GORMAN'" <lgorman1@kumc.edu>
	Copies to:      	"'Histonet@pathology.swmed.edu'"
	<Histonet@pathology.swmed.edu>

	> Linda's right.  It's the real (original) Carnoy's Fix.  I use it
and it's
	> great:
	> Acetic Acid   10ml
	> 100% ETOH     60ml
	> Chloroform    30ml
	Carle Clinic
	> Urbana, IL
	> 


	I am sure it is, but you should seriously consider finding an 
	alternative to using chloroform. This is a suspect carcinogen in 
	man and really should not be used. I wouldn't like to see this 
	fixative come back into general use, and as with mercury 
	containing fixatives,should be one for the history books.
	Terry Hacker,
	Medical Research Council,
	Harwell,
	Didcot,
	Oxfordshire, OX11 ORD
	01235 834393 x360