Re: frozen/unfixed tissue
|From:||Gayle Callis <firstname.lastname@example.org>|
You can fix frozen sections in NBF do the H&E. You may lose frozens off
slides if they are not fixed in some way. Have collected frozen sections,
immersed into NBF in a rack, and stained as much as a week later with H&E,
worked just fine, like a paraffin section. Just rinse away fixative with
water then stain, etc-----
There was discussion on this last week, check archives for more info.
At 10:57 AM 4/24/01 -0500, you wrote:
> Has anyone heard of performing H&E stain on tissue that has been
>fixed at -80 degrees Centrigrade for about 2 weeks? How would you go about
>staining for BEST RESULTS? My guess is that you would just do frozens. Oh
>the way, the tissue is kidney from a baby rat. I need to cut through the
>tissue and perform H&E. I do know that H&E's come out better once they are
>fixed,processed, and embedded in paraffin. I would rather cut tissue that
>been fixed and paraffin embedded(the results come out so much better).
>Any help is greatly appreciated.
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
404 994-4303 (FAX)
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