Cryla-

In my experience, rat brains will expand aggressively when placed on a water
bath warm enough to allow other rat tissues to relax.  My own technique is
to place the brain sections on the waterbath and have the slide I will pick
them up on ready.  I then pick up the section within seconds (<5) of placing
the tissue on the waterbath.  With practice, this is not as difficult as it
may sound.

Good luck!

Joe

Joseph A. Saby, BA, HT(ASCP)
Drug Safety Evaluation
Pfizer Global Research and Development
2800 Plymouth Road
Ann Arbor, MI 48105
Phone: (734)-622-3631
FAX:   (734)-622-3866
E-mail: joseph.saby@pfizer.com


-----Original Message-----
From: Tony Henwood [mailto:AnthonyH@chw.edu.au]
Sent: Sunday, April 22, 2001 11:10 PM
To: cryla@hotmail.com; histonet@pathology.swmed.edu
Subject: Rat Tissue Processing -Reply


Cryla,

Try turning the temperature down on your waterbath. It may be too hot.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> C H <cryla@hotmail.com> 21/April/2001 07:22am >>>
Hello, All

I wonder if any of you have a protocol that you wouldn't mind sharing for

auto-processing rat CNS tissue for paraffin embedding.  I am a novice at 
working with paraffin,using a Leica TP1050 and so far I have not found
the 
right combination of steps to prevent the tissue from overly expanding
and 
separating from the paraffin in the water bath.

Any assistance would be greatly appreciated.

Cyrla
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