EDTA and TTF-1

From:"Prada Puentes, Carlos" <Carlos_Prada@canalejo.org>

Dear "Histonetters",

I need your help again. I use EDTA buffer, PH 8 and pressure cooker with
some monoclonal antibodies, but when I use this buffer in endoscopic
samples, tissue looks as "burned". If I use EDTA with bigger samples this
problem does not appear. Do I have to reduce time of pressure cooker or what
do you think I must do?.

Thank you in advance

Dr Carlos Prada Puentes
Servicio Anatomía Patológica
Complexo Hospitalario Juan Canalejo,
Avda as Xubias 84, 15006 A Coruna, Spain
Phone 0034-981178069
Fax 0034-981178036

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