Dear Jian Ma

Did you run a dilution series on your primary?  If not, try it.  Run a
doubling dilution series far enough out so that you get no signal
whatsoever.  Work backward from there.  That usually clears up background
for me.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Thu, 12 Apr 2001, jian ma wrote:

> 
> Hi, all:
> 
> I got lots of background in oral epithelium using ABC method. I stained
> frozen section with a rabbit poly-clonal anti-human antibody. The
> antibody was not commercial now. I have applied high dilution 1 to 20
> goat serum for1 hour for blocking. The color was developed in DAB for 30
> second to 1 minute, The background staining in the epithelium still can
> be seen. It was said that rabbit serum, but not mice and goat, contains
> environmental antibodies to human epithelium or Fossman antigen reaction.
> Is it impossible to get rid of background?
> 
> Any suggestions will be very thankful.
> 
> Jian Ma
> 
> 
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