Alk phosphatase on bone

From:Gayle Callis <>

Theory and Practice of Histotechnology, Sheehan and Hrapchak pp 94-95,
Second Edition has discussion on EDTA decalcified bone/paraffin sections/AP
staining.  Although an older book, the discussion on enzyme staining of
decalcified bone for alk phos AND acid phos is excellent.  This is probably
discussed in other texts/literature as well.  What decalcifier used is
critical for both of these enzymes. 

Did you restore alk phos enzyme activity with magnesium chloride?  EDTA
decalcified bone must be treated with 1% magnesium chloride for 2 - 6 hours
or longer to restore enzyme activity. 

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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