RE: storage of antibodies
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From: | "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> |
To: | 'RUSS ALLISON' <Allison@Cardiff.ac.uk>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain |
Russ,
speaking from my own experience at the Armed Forces Institute of Pathology,
Washington D.C., we stored everything at -80 for years. When I left AFIP in
1991, we were still using antibodies frozen from 1982. Some antibodies had
to be re-titered because they became more concentrated. We froze the
antibodies in 10 ul aliquots. We took out what we needed out of the freezer
to avoid the freeze-thaw cycle. We never had a problem.
Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas
> -----Original Message-----
> From: RUSS ALLISON [SMTP:Allison@Cardiff.ac.uk]
> Sent: Wednesday, April 19, 2000 3:38 AM
> To: histonet@pathology.swmed.edu
> Subject: Re: storage of antibodies
>
>
> Are there any contra-indications to storage at minus 80?
>
> My thoughts have always been that the lower the temerature, the
> slower the reaction - any reaction. (Don't get sidetracked!!)
> We are talking here about chemical reactions in the broadest
> sense and it is they which cause antibodies to "go off", meat to rot,
> reagents to "age" and bugs to grow.
> The lower the temp., the more these reactions and these outcomes
> are inhibited.
>
> Problems are caused by:
> 1 Repeateated freezing and thawing - issues well rehearsed in
> these columns.
> and
> 2.The rate of thawing, and the example I always give is red blood
> cells are not lysed by freezing but they are by inappropriate
> thawing (generalisation, OK?)
>
> So, do some antibodies NOT need storage at minus eighty
> because they "go off" more slowly than others, or because they
> thaw "better" from minus 20 than from minus 80?
>
> Is my premise that everything can be stored at minus 80, but only
> some things at minus 20 right or wrong.
>
> Is my premise the lower the temp the better right or wrong - given
> the strong caveat about thawing.
> Just curious.
> Russ
>
> p.s.Did you hear about the vicar and the actress on the hospital
> ethics committee?
>
> Russ Allison,
> Dental School
> Cardiff
> Wales
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